Transdab
Transdab is a transglutaminase substrate database containing 506 entries, the substrates for several transglutaminase types such as FXIIIa, TG1, TG2, TG3, TG5 and TGM.
Our aim was to generate a structural database of translgutaminase substrate proteins which provides information about the microenvironment of reactive and non-reactive glutamine and lysine residues. There is an entry for each substrate protein which includes the substrate protein's name, the determination type (in vitro/in situ), the source organism where it was studied and its intracellular/extracellular localization.
Also there are specified the reactive residues and for literature reference there is a PubMed identification code given with a link to the relevant PubMed Abstract. The Expasy/TrEMBL entry is also accessible and there is a small part of the sequence given, with reactive residues in italic, to ease the database searches.
The structural information are represented by a picture gallery of reactive glutamine and lysine residues and video image of the rotating molecule, all drawn with VMD using the structural information from crystal structure files deposited in protein databank RCSB PDB. For color codes, please click here. The crystal structure is also accessible via link to the RCSB PDB through the PDBID. The surface accessibility data obtained with ASAView web server for those substrate proteins where the modification site is known and the crystal structure contains these residues is available in .pdf and text format. For intrinsic disorder prediction there is a link to one of most widely used predictor IUPred.
Transglutaminase review articles
Lorand,L. and Graham,R.M. (2003) Nat Rev Mol Cell Biol. 4, 140-56
Fesus,L. and Piacentini,M. (2002) Trends. Biochem. Sci. 27, 534-539
Griffin,M., Casadio,R. and Bergamini,C. (2002) Biochem. J. 368, 377-396
Transglutaminase sites of interests:
Acknowledgements
We thank to Istvan Andrejkovics, Gyorgy Fenyofalvi, Gabor Zachuczky and Peter Bagossi for their help. This work was supported by grant from the ESF Protein Cross-Linking - The ESF Transglutaminases Programme (PCL)
